Malay Kumar Sannigrahi

5 Questions 8 Answers 0 Followers

Questions related from Malay Kumar Sannigrahi

Any recommendations for an anti-HA tag antibody for highly unstable tagged protein IHC in mouse epithelial tissues?

I wish to a stain a HA-Tag protein by IHC from cell-lines implanted subcutaneously in mice. The protein is very unstable by nature and expressed at low levels. Is there any good well-tested...

26 October 2020 6,169 3 View

Is there any way to dissect the dynamics of mitochondria in formalin fixed tissues ?

Mitochondria is a dynamic system when oxidative-reductive processes are concerned. As per I know mitochondrial number or fission/fusion processes can be detected by TOMM20. However, I was...

19 February 2019 6,396 3 View

Microarray analysis for differential expression of miRNAs gives us quantitation of total miRNA (pri-miRNA + mature miRNA) or mature miRNA only??

During analysis of microarray data of our samples and verifying them with qRT-PCR, we observed deviation in data. For example few miRNAs which were down-regulated by 2-3 fold were found to have no...

01 February 2017 947 7 View

What is the best technique to differentiate pri-miRNA from mature miRNA??

I have been working on differential expression of miRNA by qRT-PCR. I wanted to quantitate the amount of pri-miRNAs and their mature miRNAs in my cell lines before and after treatment. Is there...

31 January 2017 10,084 3 View

How to differentiate between bioenergetic (ATP producing) and antioxidant capacity (NADPH producing) of mitochondria for its role in certain function?

Bioenergetic functions of mitochondria is generating energy currency of the cell. Antioxidant capacity provides protection from oxidative stress and cell homeostasis. However how to meaningfully...

01 January 1970 869 2 View