Malay Kumar Sannigrahi

3 Questions 6 Answers 0 Followers

Questions related from Malay Kumar Sannigrahi

Any recommendations for an anti-HA tag antibody for highly unstable tagged protein IHC in mouse epithelial tissues?

I wish to a stain a HA-Tag protein by IHC from cell-lines implanted subcutaneously in mice. The protein is very unstable by nature and expressed at low levels. Is there any good well-tested...

26 October 2020 6,117 3 View

What is the best technique to differentiate pri-miRNA from mature miRNA??

I have been working on differential expression of miRNA by qRT-PCR. I wanted to quantitate the amount of pri-miRNAs and their mature miRNAs in my cell lines before and after treatment. Is there...

31 January 2017 10,011 3 View

How to differentiate between bioenergetic (ATP producing) and antioxidant capacity (NADPH producing) of mitochondria for its role in certain function?

Bioenergetic functions of mitochondria is generating energy currency of the cell. Antioxidant capacity provides protection from oxidative stress and cell homeostasis. However how to meaningfully...

01 January 1970 835 2 View