What are the specifications of placing an antenna on an EBG structure in CST or HFSS?

Hello Happy New Year , I wish the best for all of us and our kind planet Earth in 2021 OK, I designed an microstrip patch antenna on CST and now i want to use EBG structure to enhance its...

31 December 2020 5,387 9 View

How can we determine equivalent circuit model of an antenna using CST and ADS?

hello everyone i hope you all be safe and sound during quarantine! OK i designed an antenna (MPA) in CST. now i want to determine its equivalent circuit model by ADS. is it possible? and how...

21 November 2020 688 11 View

Is there any specific boundary or excitation for an antenna with EBG structure in CST or HFSS?

hello everyone i designed an antenna and also a EBG structure to improve its behavior in both CST and HFSS but i'm not sure what kind of boundary i should use to get reasonable results. i try many...

03 September 2020 3,079 8 View

Is it good to have negative gain for our antenna??????

hi everyone. I've been reading a research letter about wearable antennas for biomedical application. and then in the abstract it said, the exact words are,"The structure achieves very good...

21 July 2020 9,175 23 View

Can anyone recommend a free software (not Mr. Bayes) to combine different molecular data sets?

I have nuclear and mitochondrial sequences each arranged separately in fasta format and I need to have a combined format of those to conduct population genetics and phylogenetic analyses. Thanks,

25 February 2016 1,234 5 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View