Hi everyone
I prepared a 5mM stock of tamoxifen citrate dissolved in DMSO but when I supply 10uM of it as said in the datasheets, it didn't cause cell death within 48 h. What's the problem?
I am working on pyridylamine compounds as nucleophiles for a reaction. During the work, I encountered an amine which is in the form of a Hydrochloride salt. To perform my reaction, I need to...
26 December 2023 3,310 1 View
I used spectrophotometry for the bacteria's solution. after some calculation according to the related table, the population of bacteria become 2.7 * 10^8. I wanna dilute it to 10^8 population for...
17 September 2023 6,913 2 View
i need a some Reseach and book for calculate of load and torque in shaft propeller and where we can see a damage or failure what information i need find ??
18 August 2023 1,563 0 View
My setup is a two-chamber reactor. The anode is Pt/C coated carbon paper and the anolyte is a mixture of 5M NH3 solution + 1M NaOH solution. The cathode is Pt/C coated carbon cloth with a coating...
18 April 2022 8,312 0 View
18 April 2022 795 2 View
Dears As you can see in attached photo, for a U-slot patch antenna the dimensions are depicted. Is there any mathematical formulas to calculate the exact position and dimensions of the slot? I...
03 March 2022 739 3 View
i want simulating a phase modulator by lumerical interconnect but i do not know how i can this for an a optical system.
03 November 2021 9,154 4 View
hi when I simulate my antenna (for example in CST), can I talk about surface wave excitation based on antenna's radiation pattern? how to know that surface wave excited based on simulation...
13 April 2021 5,548 4 View
when I simulate my antenna for example in the frequency range of (1-4 GHz) in CST the maximum gain at 2.45GHz is 1.5dBi, but when I increase the frequency range (1-10GHz) the maximum gain at...
31 March 2021 9,496 8 View
Hello I designed an antenna in HFSS and I want to use SMA connector to excite it (as you see in the picture). I used wave port. and the solution type is Modal. and defined the radiation box...
21 January 2021 1,690 6 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View