I am looking for fluorescent dyes such as Dihydroethedium (DHE) and dichlorodyhroflourescein diacetate (DCFDA) based protocol. Is there any advantage and disadvantage of using these dye with mycobacterial samples?
The best way to determine ROS in cells post mycobacterial infection is by using fluorescent dyes such as Dihydroethedium (DHE) and dichlorodyhroflourescein diacetate (DCFDA) based flowcytometry analysis. I am hereby attaching two links for your reference
Article Mycobacterium tuberculosis Eis Regulates Autophagy, Inflamma...
Article Identification of ROS using oxidized DCFDA and flow-cytometry