Do you have the following alloys?

Dear colleagues, It will be a great help if someone can provide me small pieces (1.5 cm * 1.5 cm * 1.5 cm) of cast Al 5024 alloy and Al 7068 alloy (with and/or without T6 heat treatment. Happy to...

31 July 2020 3,593 1 View

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As you may know, blocking the receptor binding domain (RBD) of novel coronavirus could be very effective to prevent more severe effects of the inflammation. So, can a BsAb co-targeting CD8+ t...

09 April 2020 8,900 1 View

can I have a list of Indian indexed journals in pediatrics ?

Besides IP and IJP, which other indexed pediatric journals are there?

06 April 2020 8,132 2 View

How to code in VHDL to display color gradient in nexus 3 spartan 6 FPGA board?

Hello All, I am trying to display a color gradient using the Spartan 6 board. Since Spartan 6 board which I am currently using supports only 8 bits for the VGA, I am not able to display RGB...

22 March 2020 2,841 2 View

Where can I find secondary data on the relationship between organizational change cynicism and organizational commitment?

Where can I find secondary data on the relationship between organizational change cynicism and organizational commitment? So that I can re-analyze it for my dissertation. Of course credit will be...

05 February 2020 4,794 2 View

What will extinct first from earth, Water or Human?

The explosive multiplication of human has exerted a huge pressure on the natural resources on earth including the water, forest and wildlife. In return, he pushed all these natural resources on...

24 January 2020 2,759 4 View

Why ethical use of technology is important?

Technoethics is defined as an interdisciplinary field concerned with all ethical aspects of technology within a society shaped by technology.

25 September 2019 276 28 View

Any standardized protocol for staining the cells which are cultured in 3D culture system?

We are working on staining the specific proteins to check for its localization. We also working of live/dead staining assay simultaneously. Since the cells are embeded in the hydrogel, we could...

07 February 2019 5,183 5 View

Can anyone introduce a review paper about different methods of optical force calculation?

Hi, Maxwell Stress Tensor (MST) method is usually used to calculate the optical force exerted on an object. However, my question is about the applicability range of this method. When do we use...

28 January 2019 4,261 3 View

How to supplement the bamboo based industries from home-grown bamboo?

In order to curtail down the import of bamboo raw material and make self-sufficient to feed the industries e.g. Incense stick industry etc. the bamboo based raw material. What initiatives India...

16 January 2019 9,562 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View