How to compute the distance in km between two points inside the Earth?

Hello everyone. I am looking for a simple formula to compute the distance in Km between two points inside the Earth. I do not mean the usual problem between to cities (distance along the surface)...

02 February 2021 3,455 4 View

How can I quantify background noise in a bioacoustic file?

I am using Audacity to analyse the presence of birds and frogs recorded with a SM4 recorder (Wildlife Acoustic). Background noise (rainfall, wind) is an important variable to consider that can...

28 January 2021 2,989 3 View

How can I project 3D objects on 2D images??

Hello! From 3D images stacks I have recovered some objects. Now I need to project those objects on 2D for further analysis. Any idea, any software, plugin, etc?

21 January 2020 4,144 4 View

Does anybody knows if there is a metodology of acute ethanol toxicity for neuroprotection studies that uses functional foods?

I recently read an article regarding the use of melatonin as a neuroprotective agent using acute ethanol exposure for 1 day and it had good results, I want to know if exists an article using...

03 January 2020 9,709 2 View

Is there a way to normalize my western blot bands since i loaded equal volume of seminal plasma (not based on protein concentration)?

I loaded 10ul of raw seminal plasma sample + 10 ul Bolt LDS buffer +4ul Bolt reducing reagent. I did the transfer using iBlot2 dry transfer system and used One Pierce ECL protein detection kit....

06 May 2019 6,912 3 View

Is there an acceptance criteria or limit for swelling index/behavior for sustained release matrix tablets?

I am doing a project on Matrix Tablets using natural products as binders (release retardant polymer) and I came across Swelling Behavior/Index. Is this necessary? I know swelling is vital for gel...

03 December 2017 8,272 2 View

I have two solution: Solution A and Solution B. I will mix the two. Which wavelength should I use for UV-Vis Spectroscopy?

I have two solution: Solution A and Solution B. I will mix the two. Which wavelength should I use for UV-Vis Spectroscopy? Solution A is pink while Solution B is green in color. If I will mix the...

16 October 2017 6,761 3 View

In identifying microorganisms using BLAST search, what is the purpose of converting the reverse sequences to reverse complement?

I have a 27F and 1492 R primers. And I wonder why the R primers should be converted to its reverse complement.

03 August 2017 6,530 2 View

Why I have low reproducibility in my real time pcr?

Hello , I'm quantifying bacteria by real- time PCR . I use plasmids with the target sequence as standards. The reproducibility is not good, one time i have 95% of efficiency and the next day is...

18 July 2016 4,605 7 View

How I can know the degree of crosslinking of a polymer?

Is it known that there is a variation in the degree of crosslinking varying synthesis parameters, but that technique I can use to know this degree?

28 April 2015 3,119 6 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View