Ermie Mariano

4 Questions 5 Answers 0 Followers

Questions related from Ermie Mariano

How can I compare the protein expression (Western blot) in lysates from conventional meat and cultured meat?

After collecting the lysates, BCA assay immediately comes next to determine the quantity of the lysates to be used for western blot. However, the scaffolds used are plant protein-based, which...

29 January 2024 879 3 View

Has anyone experienced spontaneous C2C12 differentiation while under proliferation media (DMEM+10%FBS+1%PS)?

I have been doing my scaffold research which makes use of C2C12 to test biocompatibility. Seeding density was at 1x10^5 and 5X10^5 cells/5mm disk (0.2 cm^2) with proliferation media changed...

16 August 2023 3,574 5 View

What is the best terminal differentiation marker for skeletal muscle cells?

working on analyzing skeletal muscle cells exit form myogenic differentiation.

11 January 2023 9,495 3 View

Is there a way to normalize my western blot bands since i loaded equal volume of seminal plasma (not based on protein concentration)?

I loaded 10ul of raw seminal plasma sample + 10 ul Bolt LDS buffer +4ul Bolt reducing reagent. I did the transfer using iBlot2 dry transfer system and used One Pierce ECL protein detection kit....

07 May 2019 7,026 3 View