What is the best siRNA for silencing the collagen type I gene in fibroblasts?

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Onur Bender

Hello Susana,

I think you can use col1A1 siRNA-pool. This selection will suffice.

Susana G Guerreiro

Thank you do you recommend a brand?

I'm using the Dharmacon siRNA-pools. And I'm using the same firm's transfection reagent Dharmafect.

This link is handy. http://dharmacon.gelifesciences.com/uploadedfiles/resources/basic-dharmafect-protocol.pdf

Thank you again onur. good luck in your reseacrh.