MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How do I resolve the following error I get during the execution of WRF gen_be for creating background error covariance file?

I am trying to generate the be.dat file using the gen_be facility of WRF. I have 12 hour and 24 hour outputs from WRF at 00 UTC and 12 UTC for a month. When I try to run gen_be_wrapper.ksh on my...

15 January 2020 244 2 View

Can a non-binary sensing matrix be implemented using a Digital Micromirror Device?

In compressive sensing of images Digital MicroMirror Device (DMD) is used to implement the sensing matrix. DMD works in two states, on and off. This can implement a binary sensing matrix. How is a...

05 November 2019 2,096 3 View

Embroid bodies formation from human iPSCs?

Do anyone know is it necessary to use low attachement plate instead can I use bacterological petri dish ?

10 October 2019 5,587 2 View

Can I use organisational commitment in my paper when I am only using affective commitment and normative commitment?

In my paper, I am only testing the effects of affective commitment and normative commitment but not continuous commitment. I keep having a problem in my paper when I am creating a hypothesis. Can...

18 September 2019 3,942 1 View

PLS HELP! What is the best SPSS test for this research study?

Hello I have completed a study examining how training effects employees intention to remain in the organisation. Below are the relevant variables and information needed: Question 1. What is the...

09 September 2019 306 3 View

How to perform a meta-analysis for single group?

How to perform a meta-analysis for single group? Single group means you don't have a comparison group. You have estimates i.e. Mean+_SD with sample size OR events and sample size for one...

11 August 2019 5,790 14 View

Why is PCR more preferred than Gene cloning?

The motive of both cloning (not expression cloning) and PCR is to amplify the gene of ineterst. But still, why cloning is preferred over PCR and even after amplifying your gene of interest through...

04 September 2018 502 4 View

Amplification in Negative Control but no amplification in samples?

Hi, During an experiment I have conducted series of Nested PCR and getting amplification in negative control but not in sample. Every troubleshooting measures have been taken. Also, Positive...

31 August 2018 6,258 3 View

Solubility of heavy metals increases when pH decreases. But, my soil samples are of pH range 7.3-8.4.but they are found in toxic levels.Why?

i am doing research on heavy metals-its spatial distribution and its remediation techniques. The heavy metal solubility increases as pH decreases. however my soil samples are of ph range 7....

22 February 2018 8,803 18 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Why the results when using wall thickness and when using shell conduction are different in ansys fluent?

Actually, I am running cfd simulation for a heat exchanger which has two fluids one hot and other one is cold fluid. there is a solid domain between them, which I removed and instead I have used...

01 March 2021 9,537 2 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View