What is the best RT enzyme for real time quantification of mRNA?

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Rakesh Ganji

For me, Superscript III from invitrogen worked well.

But there are many potential RT enzymes from various companies.

Andrea Lapucci

For me, Iscript from BIORAD is the best.

Ying Henderson

Tried Bio-rad one before, did not work for me. I am using Superscript II from Life Tech now. Superscript III may be better than Supercript II becasue it uses high temperature (50o vs. 42oC), but I have not tried it yet.