Rakesh Ganji

4 Questions 26 Answers 0 Followers

Questions related from Rakesh Ganji

Can one deduce translation status of the cell by ribosome/polysome isolations on sucrose gradient?

I have fractionated ribosomes/polysomes using 10-50% sucrose gradient from HEK-293T cells with and without a treatment. I see the typical peaks of Abs260 for 40S, 60S, 80S, and polysomes under...

03 November 2016 1,620 1 View

How do I solve my problems with intracellular staining in my flow cytometry experiment?

I have performed FACS for a protein by intracellular staining using the antibody specific to my protein. I have kept all the possible controls (unstained, isotype control and also primary and...

22 May 2015 8,625 6 View

How can I set biological replicates for LC-MS/MS analyses?

Hi, This is with regards setting up biological replicates for LC-MS/MS. Please help me with the approach. Say there are two conditions A and B. I make proteins for these two conditions from three...

04 December 2014 4,518 7 View

Can anyone please suggest a simple spectrophotometric method to measure RNase?

I have a protein whose probable function is RNAse. I have confirmed this by incubating the RNA with protein and loading the mixture on RNA gel. What I require now is a quantitative method to...

25 April 2013 8,945 2 View