Rakesh Ganji

3 Questions 17 Answers 0 Followers

Questions related from Rakesh Ganji

How do I solve my problems with intracellular staining in my flow cytometry experiment?

I have performed FACS for a protein by intracellular staining using the antibody specific to my protein. I have kept all the possible controls (unstained, isotype control and also primary and...

22 May 2015 8,580 6 View

How can I set biological replicates for LC-MS/MS analyses?

Hi, This is with regards setting up biological replicates for LC-MS/MS. Please help me with the approach. Say there are two conditions A and B. I make proteins for these two conditions from three...

04 December 2014 4,454 7 View

Can anyone please suggest a simple spectrophotometric method to measure RNase?

I have a protein whose probable function is RNAse. I have confirmed this by incubating the RNA with protein and loading the mixture on RNA gel. What I require now is a quantitative method to...

25 April 2013 8,902 2 View