Hi Nese,

As the DNA tends to be cross linked in fixed samples the qiagen kit will not extract high quality DNA from your samples. I have tried and failed!

I found a qiagen kit which may be of use:

https://www.qiagen.com/us/shop/sample-technologies/dna/dna-preparation/qiaamp-dna-ffpe-tissue-kit/#orderinginformation

Note that you will recover less DNA than you would if the sample had not been fixed. However, using this kit you should extract clean DNA suitable for down stream procedures. I hope this has helped!

proteinase K salt method or ready to use QIAmp DNA extraction kit is a best option for PFA

Hi Nese,

I wanted to ask you, which method did you ultimately use ?

Best

Delphine