Nese Erdem

5 Questions 8 Answers 0 Followers

Questions related from Nese Erdem

What is the best protocol to isolate DNA from PFA fixed cells?

Hello everyone, I have to isolate DNA from some samples which were fixed with 4% PFA and then sorted. I am not sure, if PFA fixation affects the efficiency of DNA isolation or not. So the question...

31 May 2016 9,135 3 View

How can I detach murine BM-MSCs from the culture dish?

Hello everyone, I am having difficulties in detaching BM-MSCs. First I have tried using Trypsin-EDTA (0,25%) but even after 20 min only half of the cells were detached. Now I am using TrypLE...

19 August 2015 4,772 18 View

What is the best time to split MSCs isolated from BM?

Hello everyone, I am isolating MSCs from mouse bone marrow and I want to know the best time to split freshly isolated cells. It has been two weeks now and the cells did not reach 80% confluency...

08 July 2015 7,527 5 View

What is the best seeding densitiy for freshly isolated mouse bone marrow MSCs?

I will be isolating MSCs from BM of 6-8 week old mice. I am planing to deplete the hematopoietic cells, endothelial cells and erythrocytes using magnetic beads against CD45, CD31 and Ter119...

24 April 2015 5,772 12 View

Is anyone familiar with intra bone marrow transplantation of mesenchymal stem cells?

Does anyone have any experience with intra bone marrow transplantation of MSCs? Are they still viable after the transplantation and do you have any idea about the recovery rates? I am new in the...

16 February 2015 4,598 3 View