I am more expert as clinician than as researcher, anyway, as editor in chief of World Journal of Transplantation I received the enclosed paper that probably may help you
Thanks a lot for your generosity in sending this article.I really appreciate your kind support .
I should extract urinary cells but this article is removing all the cells in the first round of centrifuge. I use the supernatant to do ELISA technique,But the part in which I need help, is the best protocol to extract cells from urine samples.
I found a really interesting article related to my thesis and It helped me to know how to extract cells from urine.Now I m extractind RNA from cell pellets .As mentioned in this article and several related articles too, the best kit to extract RNA from urine cells is Qiagen.But we have ordered mirvana Kit from ABI.I thought it should be as well.
We did extraction and we had alcohol contamination .
This is our results from the best round of extraction :
260/280 : 1.86
260/230 : 1.02
cons : 88.6
I need help to find my pitfalls or maybe the best way to manipulate the protocol to remove alcohol.
Below are the article I mentioned as the best answer to my question and mirvana protocol.