HUVEC are hard to transfect cells. The traditional expression system will not work. I would suggest to use Lentiviral system. For detection, WB would be enough.

Thanks Muhammad, but we don't have hood for virus work, Do you suggest to another human cell line?

Hello

Use one of these lipofectamine 2000 and  electroporation using nucleofector from lonza.

see the links for protocols steps .

Good Luck 

http://www.lonzabio.jp/catalog/pdf/ri/I458.pdf

http://cardiovascres.oxfordjournals.org/content/35/3/522

http://www.molecularinfo.com/MTM/K/K1/K1-1/K1-1-12.pdf

https://www.researchgate.net/post/What_is_the_best_way_to_transfect_HUVECs

http://www.nature.com/protocolexchange/protocols/2096

Thanks alot Every body for your help