I have suspicious of contamination in my cell culture flasks but I am not sure. at the same time, I need to check my medium whether is contaminated with bacteria or not. so what is the most practical and effective way to detect contamination not only in the flasks but also in the medium?
sincerely
Hello,
The simple method to check for bacterial contamination is by checking for turbidity both in the RPMI medium as well as in the culture flask.
If you suspect contamination and turbidity is not visible, you can streak a few micro litre of RPMI medium or the medium from the flask on to the LB agar plate, and incubate the plate in the incubator at 37 degree C overnight. If there is bacterial contamination you will find bacterial colonies on the plate the next day.
Best.
You can see the fungal contamination with your naked eye. However, bacteria (such as Mycoplasma) can be seen by 20X or 40X under inverted microscope.
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