Experimental autoimmune encephalomyelitis (EAE, sometimes also called allergic encephalomyelitis) is considered the closest model of human multiple sclerosis, though it only mimics certain aspects of the disease and there are considerable strain-to-strain variation in mice. Depending on the strain, the disease can be induced actively by immunization with myelin peptide (MOG, PLP or such) or the whole protein in combination with complete Freund's adjuvant (CFA); or passively by transferring activated T cells (EAE is considered a predominantly CD4 T cell-mediated disease). Theiler's murine encephalomyelitis virus (TMEV), a picornavirus, also induces a form of EAE in mice.
What is the best strain to induce Experimental autoimmune encephalomyelitis to mimic human MS?
I completely agree with Anil. C57BL/6 females (between 8-12 weeks) are perhaps the best for EAE induction with MOG/pertussis toxin. Interestingly, I found that the gender difference is not very prominent for C57, both male and female mice showed 100% incidence (I think this has been reported before). However, the whole disease curve is shifted to the left for female mice (not a very huge difference, but it is there) ...In my hand, the females show earlier onset and reach peak earlier. For some other strains, males don't show appreciable EAE signs at all.
Hi guys,
I have problem inducing EAE in mice. We did not see any score even after day 15. We use C57BL/6 strain and we used MOG 35-55 (300 ug) with CFA containing, 10 mg/ml of M.tb, followed by 300ng of pertussis toxin on day 0 and day 2. Do you have any suggestions? Should we increase our dosage? It will be great to know the suppliers too. The MOG we used was purchased from genscript and Pertussis toxin from sigma. Please help.
Thank you
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