I am working on the degradation capacity of naphthol by 6 bacteria isolated from wastewater which are : Pseudomonas aeruginosa , Pseudomonas Putida , pseudomonas fluorescens , Bacillus thuringiensis , Citrobacter freundii and Escherichia coli but I want to know which is the best composition of the minimum medium for the screening of these bacteria .
Thank you in advance.
Are you wanting to see if they can use it as their only carbon source? Given the large variety of species you are testing you might need more than one media formulation. Minimal medias usually use ammonium sulfate as the nitrogen source, your carbon source of choice (naphthol in this case), species specific subsets of the 20 amino acids, trace minerals like iron, copper, zinc and magnesium (some species require more) and vitamins which are also species specific.
Screening for what? Utilization of naphthol as a carbon source?
A fast scholar search will result in many results
https://scholar.google.co.il/scholar?q=screening+naphthol+degrading+bacteria&hl=iw&as_sdt=0&as_vis=1&oi=scholart
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