How to design a boost converter for a mppt controller?

I have a doubt on how to select the inductor and capacitor value when I want to implement MPPT controller. This is because we always take a maximum output voltage and current but in this case, we...

28 February 2021 3,637 2 View

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Hello, I am using Coulomb 3.3. When I try to use the option "Build input from CMT or focal mech. data" I have the next errors: Unrecognized function or variable 'deg2utm'. Error in distance2...

18 January 2021 4,487 4 View

How do you prepare the 4X Laemli sample buffer for use?

Hi, I am preparing to do a western blot and I need to add 4X Laemli sample buffer to my samples before adding them to the gel and I had a few questions (I am using the Biorad 4X Laemli sample...

13 October 2020 9,485 1 View

Does anyone have experience with movement-based kernel density estimation (MKDE)?

Hello, I have mapped the locations of several individuals of a certain species of bird. I have the GPS coordinates, the date and the time data every half hour for several consecutive days and I am...

26 September 2020 3,712 2 View

A white mass inside the head of a cretaceous ant. What can it be?

I have a small piece of Burmese amber with a worker of the stem ant Gerontoformica sp. When focusing the head using incident light, it can be seen a white bilateral structure with several lateral...

26 August 2020 9,688 10 View

How can I generate TWDP random data?

Hi, I'm currently trying to understand the "Two wave with diffuse power" (TWDP) probability density distribution and test a method of moments estimator. However, I have a problem generating data...

20 August 2020 674 3 View

Do you think the teacher should switch roles with the students while teaching a foreign language through TIC?

There are some theories, as Pelgrum and Law (2003) suggested that it is necessary to make a switch between the teacher and the student roles. I mean, the student should be also the active role in...

15 July 2020 5,169 15 View

About Fortran and defining the units?

Hi to all. I am doing a large set of calculations employing as input files called 1.log, 2.log, 3.log,......, 300.log. Usually I use this definition for the input file: OPEN(5,FILE='...

12 July 2020 5,027 12 View

What is happening to my multiplex PCR?

I am developing a multiplex PCR assay to identify three strains of bacteria (A, B & C) of which two of them (A & B) are very close-related amongst each other. Two pairs of primers were...

27 May 2020 8,824 6 View

How do I detect primer dimers in an electrophoresis gel? and how to avoid them in a PCR reaction?

Hello! I am investigating plant-pollinator interactions by means of metabarcoding of pollen loads. The approach we want to use is dual-indexing in a two step PCR process. We are using four...

04 May 2020 7,455 6 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

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01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View