What is the best method transplant macrophages into mouse lung after original alveolar macrophages are depleted? Do I have to use BMDMs?

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How can I speed up the growth of single MCF-7 clones after cell sorting?

I recently did a single cell sort with MCF-7 cells to create my CRISPR cell line. I sorted single cells in a 96-well plate in MEM media with 10% FBS, 10ug/mL insulin, and 1mM sodium pyruvate. It...

28 October 2023 6,320 1 View

What should be the minimum organic carbon content in soils for Pyrolysis - GC MS analysis? Is there any pretreatment or sample preparation procedure?

I am working on soil organic matter characterization, what should be the minimum organic carbon content in soil? Which particular column should I use in GC MS ? Is there any national facility...

04 June 2022 1,822 0 View

What does Gamma 6 , Gamma7 and gamma 8 in HgTe band structure represents?

Band inversion in HgTe is explained on the basis of band ordering of Gamma 6, Gamma 7, and Gamma 8 positions. But what do these 6, 7 and 8 represents? can anybody explain briefly how can we...

15 May 2022 5,722 7 View

How to prepare data file for POPGENE Software?

If we want to study the population genetics/population structure of 30 genotypes by employing SSR markers. How we have to prepare input data file of SSR markers data to analyse in POPGENE Software.

16 May 2021 3,650 5 View

What is the difference b/w a sputter target directly purchased from Alfa, etc., and a circular plate wire-cut from the thick plate of same purity?

Previously, we used to get a thick metal plate (similar purity as the sputter target) and wire cut it make as circular target of 50 mm dia. In this way, one gets 4 sputter targets with the same...

17 August 2020 9,025 3 View

Can any one help me in getting the list of scopus journals for MRI image processing using Deep Learning?

Can anyone help me in getting the list of Scopus journals for publishing MRI image processing using Deep Learning? and also let me know how can I get an authorized Scopus list where i can publish...

29 May 2020 501 2 View

How many number of primers of a gene to be used for its expression analysis using RT-qPCR?

Do we get any differences in assessment of gene expression using primers from many exons of a genes? In general, it is done using single optimised primer in Realtime PCR. What happens if two...

22 March 2019 7,545 3 View

Does solvent plays an important role in free radical polymerization? If yes how and if no Y?

what are the factors which will be effected by different solvents in free radical polymerization?

19 September 2016 2,869 4 View

How do I estimate hertability for the traits from the lines evaluvated in Augmented Block Design?

Is it possible to estimate environmental variance as the treatments are unreplicated in ABD. Please provide the SAS code for the estimating hertability from the lines evaluated in Augmented block...

18 January 2016 562 2 View

Can anyone suggest the source for Human papillomavirus positive controls (all high risk types)?

I am working on human papillomavirus, one of my objectives in the study is to standardize multiplex PCR for common high risk types. I am trying to get positive controls for the same but only HPV...

16 December 2015 2,484 0 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

Human macrophages cell surface markers?

I have a question about human macrophages cell surface markers.I want to stain the unpolarized human macrophages for flow cytometric sorting without permeabilizing them. It is because I need to...

29 July 2024 236 2 View

Frontiers in kidney transplant research: What are the Challenges and Prospects?

Research Questions 1. What are the most effective strategies to prevent organ rejection post-transplant? 2. How can we improve long-term outcomes for kidney transplant recipients? 3. What...

20 July 2024 9,468 1 View

Fixation Problem with cancer cells?

Hello everyone, I work with polyacrylamide hydrogels, which I fictionalise with sulfo-sanpa and collagen I to then plate lung cancer cells. The problem is that I lose a significant amount of...

19 July 2024 3,906 0 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

FACS markers to characterize rat bone marrow-derived macrophages?

Dear all, I am looking for FACS markers to characterize my rat bone marrow-derived macrophages (M0). They were differentiated from monocytes with M-CSF. I am considering to use CD11b/c and...

15 July 2024 4,615 1 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

Does egg white interfere with any histology staining?

I have mouse lung tissue which is mounted with egg whites.I need to stain my tissues with PAS and HE. Can egg whites interfere with these stainings?

02 July 2024 8,261 0 View