What is the best method to separate degraded amylopectin fractions by gel chromatography?

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Greg Cote

It depends on the size of the fractions. Bio-Gel is suitable, but the exclusion limits of each type of Bio-Gel range from about 2kDa to 300 kDa. Do you have any estimates of the size range of your products?

Heba Abdelwahab

I can see. I found it in mane research papers. but I am just making sure if there is any other method before I start working. it is not available for me know and may be take time to be delivered. any way I need high Mr fractions.

please if you have a detailed method, it would really help .

thank you

I appreciate it

Alexander Mikhailovich Zakharenko

If You no need to obtain a big amount of this products Shodex Asahipak NH2P-50 4E, or another amide column is very good to control oligosaccharides, or even C8 column is suitable if the molecular size is small