Heba Abdelwahab

9 Questions 17 Answers 0 Followers

Questions related from Heba Abdelwahab

How can I quantify my product in HPLC?

For my experiment, I am running an enzymatic reaction, if I need to quantify the product but I don't have a standard or extinction coefficient at a specific absorption. Can I use the decrease in...

20 January 2016 9,581 5 View

Can normal UV spectrophotometer cause enhancement of a compound Spectra?

HI I was wondering if the light of UV-vis spectrophotometer can enhance the transmitted light (increase the absorption at specific wavelength of a compound when measured over time? I have this...

20 September 2015 9,874 11 View

Any advice on enzyme denaturation and spectral change upon measuring kinetics by UV-spectroscopy?

I am working with a flavin-dependent enzyme, when I am trying to measure kinetics for the enzyme over long time using UV-spectrophotometer. I can realize that the enzyme starts to precipitate all...

06 September 2015 1,873 5 View

Enzymatic substrate inhibition fitting?

I was wondering what is the best way to fit the enzymatic reaction rate when I have substrate inhibition. I tried using Kaleidagraph susbtrate inhibition equation, it is showing a very good fir...

19 August 2015 9,762 12 View

How can I calculate the catalytic efficiency values?

If you have an enzymatic reaction with substrate inhibition and you want to get the catalytic efficiency. What is a suitable way to calculate the value and would that be a real number? Is dividing...

26 March 2015 2,513 3 View

How can you prepare your model of protein before going through Gromacs and Autodock programs?

Reference(s) would be great. Thanks!

22 September 2014 5,158 11 View

How can I dissolve starch to use with the same concentration (i.e: without centrifugation) you prepared, to be applicable with enzyme activity assays?

Dissolving starch (soluble one) completely

23 July 2014 2,963 4 View

What are the most convenient methods for estimation of Glucose-1-phosphate among other sugar fragments?

Is it enzymatically, or by HPLC and mass spectrometry, or are there other methods?

31 December 2013 1,582 3 View

What is the best method to separate degraded amylopectin fractions by gel chromatography?

I need the fractions as pure as possible.

09 December 2013 5,736 3 View