What is the best method to quantify the concentration of a fungus without spores, eg Rhizoctonia solani?

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How do I make the fungal culture having the spore concentration of 4.8×10⁶ spores/ml ?

Also Explain me how much amount of fungal biomass should I make in order to achieve the above concentration?

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Zheng Wang

probably "dry mass" is the best way, depend on what you do next.

Nanbiao Long

you can grind it and then measure the OD600 to quantity the concentration

Rosa Caiazzo

why do you need to quantify it for? if you need to make an inoculum and start from the same " concentration" you can cut plugs from a PDA cultures with a 5-mm-diameter cork borer and used as inoculum