How to promote sporulation of endophytic fungi after isolation and purification of the
To induce stress in fungal colonies or culture plates, various conditions can be applied:
01. Nutrient Limitation: By reducing the typical amount of culture media (e.g., PDA/MEA) per plate, nutrient limitation can be achieved, prompting the onset of sporulation.
02. Physical stress can be induced in fungal colonies through various methods:
- Needle Damage: Using sterilized needles to damage the colony can provoke stress, prompting sporulation.
- UV Exposure: Exposing fungal colonies to UV light for a short duration is another method to induce stress, triggering sporulation.
- Additionally, fungal colonies can be subjected to stress by being kept with double-sterilized toothpicks, which can also facilitate sporulation.
03. Temperature Stress: Altering the temperature beyond the usual range can induce stress. Both lower and higher temperatures than the usual maintained temperature can facilitate sporulation.
It depends on the type of endophytes. I had mild success by plating some fungi with their natural substrate sterilized (leaves). I think it's always a good to give some hard surface to the fungus, because some of them are definitely thigmotactic.
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