What is the simplest way to implement data encryption for Arduino project that communicate through Bluetooth ?

I have developed a project based on Arduino that communicates through Bluetooth with Android application, I want to enhance its privacy through encryption so that data should be prevented up to...

07 January 2021 7,933 5 View

How to configure wifi password and SSID on NodeMCU ESP8266 through wifi direct instead on hardcoded credentials?

While working on IoT projects I mostly use NodeMCU ESP8266 firmware for IoT Implementation. For coding, I use Arduino IDE in which for connectivity with wifi router we define hardcoded variable...

07 January 2021 6,688 3 View

What criteria should be followed in designing schools?

criteria should be followed in designing schools

31 December 2020 682 0 View

Could anyone suggest the profiling packages in python which similar to profvis package in R?

I am currently working on the benchmarking of the tools and programs developed for handling biological data, I have used R-programming wherein I used Profvis for the R based tools. However, there...

15 December 2020 5,764 3 View

How to plot a Slope gardient Map of Bathymetry?

In order to find out the area/location prone to submarine landslide, plot of slope gradient map of Bathymetry is very helpful. Guide me, how to plot of slope gradient map (in detail/steps) of...

05 November 2020 8,144 4 View

Anyone who wants to work or currently working on federating learning(Distributed Machine Learning)?

I am a Masters Student of Computer Science and Technology at Dalian University of Technology, currently working on fairness/uniformity in highly heterogeneous network of federated learning.

27 October 2020 5,661 3 View

Can you please tell me which problems ,in general, the data mining can solve?

Data mining is a process of discovering patterns in large data sets involving methods at the intersection of machine learning, statistics, and database systems.

26 October 2020 4,364 5 View

What are suitable databases for IoT?

For Internet of things (IoT) which databases are suitable or desigining. I want to know the details of about it.

26 October 2020 2,499 15 View

What are the advantages of a computer network ?

Computer network is a group of computers that use a set of common communication protocols over digital interconnections for the purpose of sharing resources located on or provided by the network...

05 October 2020 8,105 25 View

Homemade liquid fertilizer, How to make it?

Dear Homemade liquid fertilizer derived from manure or organic waste, how we can make it for laboratory experiments. Is there any scientific method/ or papers to produce this product (with...

22 September 2020 3,635 8 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View