Hi all,
I am looking at performing mass-spec of human skin tissue to look at the presence of different phospholipids. Can anyone shed any light on the best protocol to use for this?
Many thanks
Dear Tara,
Lipids in tissue and fluid samples depend on several factors, the usual ones (age, gender, ethnicity, diet) and others including the extraction solvent (see ref below).Article A comparison of five lipid extraction solvent systems for li...
Recently I came across a studie that I think might be worth looking at
Article Large-scale human skin lipidomics by quantitative, high-thro...
Hope this helps,
Ana
Should the goal be a phospholipidomic profiling, classical extractions such as Bligh&Dyer are adequate. For skin biopsies, we have followed the procedure indicated in the paper enclosed. Efficiency of extraction for all phospholipid classes and subclasses was tested and found to be near 100%. This is also applicable to TG and cholesteryl esters. Phosphorylated PI and glycosphingolipids are not extracted properly and using a specific extraction method is recommended.
- Badges
- Science topic
- Similar topics
- Mathematical Sciences
- Graphs