I feel silica-guanidiniumthiocyanate DNA isolation procedure is the most appropriate one to extract DNA from fecal matter. (If you have more funding, you can use Qiagen stool kit for extraction).
Dear S Yellapu sir it is quite efficient method but comparatively expensive and sometimes fails in samples from herbivorous animals in the study(paper of Li-Cha Ma
A friend of me do it with a QIAamp Stool mini kit of QUIAGEN with some modification on the protocol and it work really well. Just look this paper in the section DNA extraction of the materials and methods for precision.
http://www.biomedcentral.com/content/pdf/1471-2148-9-68.pdf
Hi, I also used a QIAamp Stool mini kit of QUIAGEN with some modification on the protocol to extract DNA from Capercailie faeces. Worked quite ok. As far as I remember, larger quantities of the chamicals could also be bought in bottles, which made things much more cheaper.
http://www.sciencedirect.com/science/article/pii/S0006320712001930
Yes, I also used the QIAGEN QIAamp DNA Stool Mini Kit with some modifications. In the past I used Guanidine thiocyanate and definitely had more success with the Qiagen kit. I also used it on samples that would not amplify with the Guanidine method, and found some of them to work with the Qiagen kit.
Dear, actually in our Genetics there is a dissertation student whose work is on wild life forensic, this time he is standardizing the protocol for DNA isolation from faecal matter as soon as the protocol will standardize, i will tell you the best method.
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