What is the best method of DNA extraction for pyrosequencing?

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What is the recommended type of pseudopotentials for QE?

Hello dear colleagues and professors Please what is the recommended type of pseudopotentials for QE? ultrasoft or norm conserving?? And from where get all types of pseudos (us, nc, pbe,...

09 July 2024 9,541 5 View

Root Phenotypes in Arabidopsis Col-0 Under Phosphate Deficiency Conditions not showing up??

Introduction: I am conducting an experiment to investigate the root phenotypes of Arabidopsis Col-0 plants grown under phosphate deficiency conditions. Here, I describe my current methodology and...

19 June 2024 8,774 3 View

Doping/Substitution of an element in QE?

Hello dear researchers, I hope you are doing well. I want to ask you a question. I have a unit cell with 24 atoms (4 A, 4 B and 16 X) and I want to substitute X atom by another atom (for example...

28 May 2024 5,571 5 View

Is it possible to statistically associate data from two similar surveys conducted on the same population twice?

I would like to conduct a survey to assess the level of awareness students have regarding internet threats and their vulnerability to social engineering. However, I plan to conduct the survey...

25 April 2024 1,800 11 View

What is PEDAGOGICAL ASSESSMENT of students' performance?

I just want to know as there is pedagogical leadership and pedagogy is much explained, however the relationship between assessment of students' performance and teacher's employed pedagogy.

02 April 2024 1,606 7 View

How to resect the superior mesenteric ganglion in mice?

I am trying to resect the CG-SMG in mice. Although I can locate the right area as in the attached photo, I could not identify the ganglion. Any help would be greatly appreciated....

28 February 2024 4,672 0 View

Relaxations induced in natural fibers by water absorption ?

Dear researchers, I have a question please. What are the different relaxations induced in natural fibers by water absorption? Best regards,

10 February 2024 2,745 0 View

What are the key metrics used to evaluate the reconfiguration effort in reconfigurable manufacturing systems?

These may include time, cost, resources utilized, disruptions to production, etc.

07 February 2024 8,775 0 View

How to calculate sample size for rare disease?

i want to do cross sectional study in osteogenesis imperfecta patients. the prevalance is about 6.5 per 100000 live births. kindly help me how to calculate the sample size

23 January 2024 4,712 3 View

Troubleshooting "Cannot Modulate Selected Heatflow" Error in Modulated Convectional MDSC Analysis on DSC?

I am encountering an error stating "Cannot Modulate Selected Heatflow" while attempting to perform Modulated Convectional MDSC analysis on a Differential Scanning Calorimeter (DSC). Has anyone...

22 January 2024 8,671 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View