What is the general cyclic energy degradation factor for plain concrete at material level in stress strain curve?

Plain Concrete subjected to cyclic loading get reduction in its stiffness and strength with the every cycle of loading. What is ratio of energy enclosed in one hysteresis loop to adjacent...

10 February 2021 1,051 1 View

The Akaike information criterion of the random forest model ?

Please, how to calculate the number of model parameters of the random forest model to determine the Akaike Information Criterion (AIC) using this following expression: AIC = n * log(MSE) + 2 *...

27 January 2021 816 6 View

Suggest me some satellite image datasets used for crop yield estimation?

I have my synopsis presentation and i have to provide details about 2-3 datasets that I can use in my research. Topic is crops Yield estimation using satellite images. suggest me what I can write?...

13 January 2021 1,675 3 View

What is the reason of suddenly increasing the concentration while evaluating the photocatalytic performance by RhB dye degradation?

I am doing a photocatalytic performance evaluation by RhB dye degradation. I am facing a problem which is: After achieving the absorption -desorption equilibrium, I checked the concentration and...

04 December 2020 3,922 20 View

Edu assay by spectrophotometry means?

Dear all, Have any one performed edu assay by spectrophotometric means ? I'm looking for a kit for that purpose. I have Chinese kit for this purpose, I followed their protocol but the result is...

24 November 2020 1,766 3 View

Either SEM analysis are enough to justify the morphology and micro structure of activated carbons? or I should go for TEM analysis as well?

While I was discussing my nitrogen adsorption isotherms in the context of micropore, mesopores, total pore volume for activated carbon samples prepared from waste materials, I received a review...

13 November 2020 1,971 3 View

How can we measure dissimilarities between two images layout? Like alignment of button, text box, text overlapping?

I want to extract dissimilarity information of two images layout. Wanna check text, button, and text box alignment, text overlapping, and more other layouts difference. how we do that? any tool,...

25 October 2020 7,190 7 View

Any suggestions of bioinformatic tools to identify/predict the effect of mutations observed on the function of proteins?

04 October 2020 1,552 4 View

How to Determine LD50 of a test compound by Lorke's Method if your mice (mus musculus) dies at the lowest concetration i.e. 10mg/kg?

I have used Lorke's method of determining LD50 of my test drug. The lowest dose according to this method is 10mg/kg and my mice died at this dose within the 30 minutes of IP injection. Does that...

22 September 2020 9,959 3 View

How to do MTT for non-confluent cells?

Hi everyone, I'm doing MTT assay to test the cytotoxicity of 4 candidates on the BT-474 breast cancer cell line which rarely becomes confluent. started with 3K/well, 5K/well, 6.5K/well left the...

12 September 2020 3,034 8 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View