What is the general cyclic energy degradation factor for plain concrete at material level in stress strain curve?

Plain Concrete subjected to cyclic loading get reduction in its stiffness and strength with the every cycle of loading. What is ratio of energy enclosed in one hysteresis loop to adjacent...

10 February 2021 1,051 1 View

The Akaike information criterion of the random forest model ?

Please, how to calculate the number of model parameters of the random forest model to determine the Akaike Information Criterion (AIC) using this following expression: AIC = n * log(MSE) + 2 *...

27 January 2021 816 6 View

Suggest me some satellite image datasets used for crop yield estimation?

I have my synopsis presentation and i have to provide details about 2-3 datasets that I can use in my research. Topic is crops Yield estimation using satellite images. suggest me what I can write?...

13 January 2021 1,675 3 View

What is the reason of suddenly increasing the concentration while evaluating the photocatalytic performance by RhB dye degradation?

I am doing a photocatalytic performance evaluation by RhB dye degradation. I am facing a problem which is: After achieving the absorption -desorption equilibrium, I checked the concentration and...

04 December 2020 3,922 20 View

Edu assay by spectrophotometry means?

Dear all, Have any one performed edu assay by spectrophotometric means ? I'm looking for a kit for that purpose. I have Chinese kit for this purpose, I followed their protocol but the result is...

24 November 2020 1,766 3 View

Either SEM analysis are enough to justify the morphology and micro structure of activated carbons? or I should go for TEM analysis as well?

While I was discussing my nitrogen adsorption isotherms in the context of micropore, mesopores, total pore volume for activated carbon samples prepared from waste materials, I received a review...

13 November 2020 1,971 3 View

What does skewness and kurtosis in construction management refer to and what are permissible limits of these in civil engineering with references?

The question is about assessment of skewness and kurtosis to carryout a research in construction management through SPSS software. The purpose behind the question is to know the permissible values...

12 November 2020 7,114 3 View

How can we measure dissimilarities between two images layout? Like alignment of button, text box, text overlapping?

I want to extract dissimilarity information of two images layout. Wanna check text, button, and text box alignment, text overlapping, and more other layouts difference. how we do that? any tool,...

25 October 2020 7,190 7 View

Any suggestions of bioinformatic tools to identify/predict the effect of mutations observed on the function of proteins?

04 October 2020 1,552 4 View

How to Determine LD50 of a test compound by Lorke's Method if your mice (mus musculus) dies at the lowest concetration i.e. 10mg/kg?

I have used Lorke's method of determining LD50 of my test drug. The lowest dose according to this method is 10mg/kg and my mice died at this dose within the 30 minutes of IP injection. Does that...

22 September 2020 9,959 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View