How Triton-x removes SDS from SDS-Polyacrylamide gel?

I came to know about removal of SDS from Polyacrylamide gel is done by Triton-X, but what is the exact mechanism of its removal. How Triton-x does that? Thank you

03 February 2021 7,206 2 View

How can i calculate diffusion barrier of Li ion on graphene nanoribon using SIESTA?

I'm a new siesta learner, I'm working on lithium-ion batteries, I have calculated adsorption energy but i am not able to calculate diffusion barrier of Li ion. kindly help me how to calculate...

14 January 2021 2,688 1 View

How to resolve the negative peaks in RI detector ?

I am using Phenomenax rezex column with 5 mN H2SO4 as eluent flowing through the column at flow rate of 0.6 ml/min. RI temperature is 36C and column temperature is 60C. At the time of glucose...

17 November 2020 2,297 4 View

Incorrect bandstructure of zigzag ZnO ?

I'm a beginner in siesta code. I'm trying to simulate zigzag Zno structure using siesta. After optimization I found that Zn atoms form an unwanted bond which I think could be reason for incorrect...

03 January 2020 7,833 3 View

How to separate mixture of two organic compounds?

I have mixture of two organic compounds one with molecular weight 226.41g/mol and other is 166.22 g/mol. Can I use size exclusion chromatography for the difference of 60 g/mol? Kindly do let me...

04 December 2019 9,952 3 View

What is emotional intelligence?

Emotional intelligence is one of the important factors determining success of an individual. How can a person manage his own emotions and the emotions of others for achieving his/ her goals in an...

02 December 2019 6,305 8 View

How to coat graphene oxide on magnesium alloy for implant application?

implant application

24 February 2019 6,312 3 View

What are the free alternatives to Graphpad prism to generate graphs for publication?

can anyone suggest software apart from R studio? (for windows 10).

08 January 2019 6,836 2 View

Can anybody provide me matlab code for Chaotic Numbers?

Chaotic based matlab code

19 April 2018 8,600 2 View

If the paper is uploaded on arxiv, why does astrobiologist editor think it as already published? If so what is the next step for author to resolve?

I usually upload the article on arxiv before sending it to a journal to protect copy rights. If the editor does not no what is arxiv and rejects your article, then what is the next step?

15 April 2018 6,789 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View