What is the best method for tRNA isolation?

More Abu Musa Md Talimur Reza's questions See All

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Bangladesh government's reported plan to use lethal force against protesters? We need help Urgently ?

Please Help Us Urgently

01 August 2024 711 1 View

If the emission of all greenhouse gases is stopped now, will the temperature of the earth continue to rise?

Here's a serious question: If we were to stop emitting all greenhouse gases right now, would the Earth's temperature start to cool down, or would the existing greenhouse gases continue to warm the...

30 July 2024 5,123 4 View

"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students ?

"How has Leader Sheikh Hasina's government allegedly responded to student protests, including the reported killing of over 500 students, as well as arrests, custody, remand, and the involvement of...

29 July 2024 2,181 7 View

Can a photocatalytic degradation of methylene blue from red mud be pseudo- zero order kinetics?

My photocatalyst from solid waste red med. Dye is methylene blue My all parameter study is showing zero order. How to prove it further that the reaction in zero order?

29 July 2024 7,404 1 View

How to calculate pseudo order kinetics?

I know the rate law. But for my photo-catalytic experiments, first 90min is adsorption (dark) and next 150min is under UV light (total 240min). How to remove the adsorption part for calculation?...

21 July 2024 8,807 6 View

Query Regarding tRNA Annotation Order in Feature Table for NCBI Submission?

Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....

30 June 2024 8,650 0 View

List Plasmid suitable for Homology cassette in CRISPR-Cas9 protocol for Knock-In in mammalian cell line ?

Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...

25 June 2024 1,197 0 View

Is this mycoplasma contamination?

I had mammalian cancer cell line cultured and fixed for confocal imaging. While observing I found these particles around cell nuclei stained with dapi. I checked the main culture for bacteria but...

15 May 2024 9,102 2 View

Is this mycoplasma contamination?

I had mammalian cancer cells cultuted and fixed on confocal slides. I could see these small small bodies stained with dapi. The main culture flask doesnt show any bacterial growth. Is this...

15 May 2024 6,040 3 View

How do I prevent loss of my H6c7 non-cancerous pancreatic cells during centrifugation?

Everytime I centrifuge my H6c7 cells, the cells don't pellet properly. Though there is a pellet formed, the supernatant has visible particles which I believe to be the cells. I usually centrifuge...

28 April 2024 5,685 1 View

Has anyone designed guideRNA for multiple targets using polycistronic-tRNA-gRNA expression system?

I am planning to design guideRNA for multiple targets to express as a polycistronic-tRNA-gRNA expression. I would like to know whether we need to include any linker between the tRNA and guideRNA...

29 March 2024 1,055 0 View

Why does my sample show inconsistent band pattern in western blot?

Hello, I'm having a bit of trouble doing the Western blot experiment. Even though I load the same sample, the band appears inconsistently (in all the trials). (I usually store the samples at -20°C...

18 February 2024 2,863 1 View

Promega Passive Lysis Buffer for Bacterial Cells?

Are there any conditions where it would be possible to lyse gram-negative bacteria using the Promega PLB? I would need to lyse the cells to test for crude enzyme activity but I don’t have a...

30 January 2024 7,625 1 View

Protocol for overexpressing NOX4 in a mammalian cell line?

I need to overexpress the protein NOX4 in a mammalian cell line. What methods should I use?

30 January 2024 7,120 0 View

Advice for TIDE Primers?

Hi Everyone! I am currently designing primers to validate my CRISPR-KO mammalian cell line using TIDE. The primer is designed about 250bp upstream of the PAM site. Then, Sanger sequencing is...

27 January 2024 2,219 1 View