Regarding IHC, how can this method successfully express protein expression?
Quantitative immunohistochemistry has been reported. Here are a few papers.
https://www.ncbi.nlm.nih.gov/pubmed/12533529
https://www.ncbi.nlm.nih.gov/pubmed/22515559
l need some details about these sites to start my work as a freelancer, thanks.
01 February 2015 3,053 1 View
Do our results measuring mRNA gene expression with RT-PCR can reflect our results measuring protein expression by IHC? I would like to know if my results by RT-PCR should reflect that for IHC.
31 December 2014 6,944 5 View
During performance of one-step RT-PCR reaction, however the reference gene is expressed, the target gene not determined by 7500 fast apparatus, is that because the extraction purity is not enough...
06 July 2014 7,918 4 View
I am working in a project that l use both one step and two steps methods, but l would like to know which of them more useful and specific.
04 May 2014 4,374 3 View
Immunohistochemistry is a method for demonstrating the presence and location of proteins in tissue sections. Though, less sensitive quantitatively than immunoassays such as Western blotting or...
04 May 2014 9,525 7 View
Genomic imprinting differs from that of ordinary Mendelian rules of inheritance, as in the first aspect the gene is expressed by one allele from one parent and the other is silenced by imprinting;...
04 May 2014 961 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View