Regarding IHC, how can this method successfully express protein expression?
Quantitative immunohistochemistry has been reported. Here are a few papers.
https://www.ncbi.nlm.nih.gov/pubmed/12533529
https://www.ncbi.nlm.nih.gov/pubmed/22515559
l need some details about these sites to start my work as a freelancer, thanks.
01 February 2015 2,959 1 View
Do our results measuring mRNA gene expression with RT-PCR can reflect our results measuring protein expression by IHC? I would like to know if my results by RT-PCR should reflect that for IHC.
31 December 2014 6,864 5 View
During performance of one-step RT-PCR reaction, however the reference gene is expressed, the target gene not determined by 7500 fast apparatus, is that because the extraction purity is not enough...
06 July 2014 7,822 4 View
I am working in a project that l use both one step and two steps methods, but l would like to know which of them more useful and specific.
04 May 2014 4,279 3 View
Immunohistochemistry is a method for demonstrating the presence and location of proteins in tissue sections. Though, less sensitive quantitatively than immunoassays such as Western blotting or...
04 May 2014 9,455 7 View
Genomic imprinting differs from that of ordinary Mendelian rules of inheritance, as in the first aspect the gene is expressed by one allele from one parent and the other is silenced by imprinting;...
04 May 2014 866 2 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity
03 March 2021 8,890 1 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?
02 March 2021 557 2 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View