What is the best method for protein expression?

01 February 2017 4,821 1 View

Does mRNA level reflects protein level?

Do our results measuring mRNA gene expression with RT-PCR can reflect our results measuring protein expression by IHC? I would like to know if my results by RT-PCR should reflect that for IHC.

31 December 2014 6,864 5 View

Can RNA extraction purity affect our target gene expression results on RT-PCR?

During performance of one-step RT-PCR reaction, however the reference gene is expressed, the target gene not determined by 7500 fast apparatus, is that because the extraction purity is not enough...

06 July 2014 7,822 4 View

What are the benefits of using one step and two steps RT-PCR?

I am working in a project that l use both one step and two steps methods, but l would like to know which of them more useful and specific.

04 May 2014 4,279 3 View

How can immunohistochemistry confirm protein expression of selective genes?

Immunohistochemistry is a method for demonstrating the presence and location of proteins in tissue sections. Though, less sensitive quantitatively than immunoassays such as Western blotting or...

04 May 2014 9,455 7 View

How can the maternal or paternal allele be inherited as genomic imprinting? What do we mean by germ-line reprogramming of an imprinted gene?

Genomic imprinting differs from that of ordinary Mendelian rules of inheritance, as in the first aspect the gene is expressed by one allele from one parent and the other is silenced by imprinting;...

04 May 2014 866 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

No title

02 March 2021 3,060 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View