Do our results measuring mRNA gene expression with RT-PCR can reflect our results measuring protein expression by IHC? I would like to know if my results by RT-PCR should reflect that for IHC.
No. The mRNA levels that appeared abundant may yield low abundance protein. This is the result of post transcriptional regulations that affect the mRNAs before translated into protein.
Hi there,
Regulation also occurs at the translation level which means there is no strict proportionnality between mRNA level and translation product level
Sometimes yes, sometimes no. In addition to Wan-Chin's answer, you might consider influences of protein modification such as ubiquitination.
I agree with the collegues, gene expression is tightly controlled by a number of post-transcriptional and post-translational mechanisms. Thus, to confirm your RT-PCR data you should also analyze protein expression by IHC.
NO and NO and NO.
Transcription and also degradation as well as translation are different mechanisms in charge of messenger level and protein synthesis.
Some messengers even if their levels are low in a cell can be very efficiently translated in proteins (according to their structure and the cell environment, etc. ). And be aware that one molecule of mRNA might be repeatidly translated amplifying therefore the level of the proteins.
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