For lysosomal pH measures currently in the literature there are several dyes that you can use without affecting cellular function. I recomend the two that are used in the paper that I am sending you. At least in my hand they have worked fine. All depends is you need a qualitative assay or a quantitative one.
For lysosomal pH measures currently in the literature there are several dyes that you can use without affecting cellular function. I recomend the two that are used in the paper that I am sending you. At least in my hand they have worked fine. All depends is you need a qualitative assay or a quantitative one.
Dear Devrim,
I found two papers though kind of old where you can get some idea for the fluorescence probe based intralysosomal and endosomal pH measurement.
I am sharing these two papers a combined pdf.
Hope that helps,
In their review "Lysosomal pH and analysis of the counter ion pathways that support acidification", DiCiccio and Steinberg provide a nice survey of advantages and caveats of commonly used methods to measure lysosomal pH (acidotropic and ratiometric dyes). We use dextran-coupled OregonGreen chased into lysosomes to ratiometrically measure their pH. Obviously, it's a more difficult issue for endosomal pH. As this differs between the different stages and kinds of endosomes, you have to be sure that your pH sensor is in the correct compartment of interest - therefore dye taken up by endocytosis would be problematic because whatever may affect the pH may as well affect at least the time of delivery to your compartment.
Life technologies sells a product called pHrodo dextran with a molecular weight appropriate for endocytosis:
https://www.lifetechnologies.com/order/catalog/product/P10361
Essentially, the probe will increase in fluorescence as the endosomal compartment acidifies and becomes part of the lysosomal network.
They also sell it as a green color.
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