What is the best method for cell lysis in large volumes in case of intracellular enzymes?

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Some enzymes are intra cellular while some are extra cellular in microbes?Both in some cases,why?

My interest is in beta galactosidase which is intracellularly produced in bacteria and yeasts while it is extra cellular in case of fungi and some bacteria in submerged fermantations under shake...

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Can anyone please help me out with how to convert a Korean article into English???

Article is from Korean journal of Microbiology and Biotechnology.....

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Can anyone tell me the protocol for constructing a standard graph of ONPG for beta galactosidase assay?

I want to know whether ONPG is converted to ONP or directly ONP is used for the standard graph?

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Is there any other activity for beta galactosidase other than lactose hydrolysis and transgalactosylation?

I have screened actinomycetes possesing beta galactosidase activity.Ineed to know whether the enzyme is having any other activity like antibiotic activity or anti cancer or anti inflammatory...

08 July 2015 1,773 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

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Ahmed H. El-Sappah

hi, I suggest you these papers:

1- Cell disruption methods

file:///C:/Users/Administrator/Downloads/Cell%20disruption%20methods.pdf

2- Disruption of microbial cells for intracellular products

http://www.massey.ac.nz/~ychisti/CellDRev.pdf

3- A Review on Macroscale and Microscale Cell Lysis Methods

https://pdfs.semanticscholar.org/1e5a/6d01f9c938c6158d92192e42224efa064e31.pdf