What is the best method for bioanalysis of glimepiride in human plasma?

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How to count using allred method in IHC?

dear all, i would like to ask how to count using allred method in IHC, i have several paper that i have read but i have difficulties in understanding the calculation of intensitiy score, if anyone...

27 June 2016 1,697 2 View

Does anyone know the recent development of vanadyl sulfate as an antidiabetic agent?

i am planning to do a research about vanadyl sulfate as an antidiabetic agent on a mice i will learn to study in correlation with the apoptotic and proliferation in pancreatic beta cell. does...

19 August 2015 4,295 4 View

Problem with ion suppresion in HPLC-MS (curcumin, mouse plasma)?

We want to determine curcumin in mouse plasma after subcutaneous administration of some formulations. The idea was to perform the determination by HPLC-MS. However, we have been having a problem...

10 July 2023 4,113 2 View

Peptide and ionization in mass spectrometry - Which adduct ion could be formed?

My peptide is Cholecystokinin (CCK8), MW=1142.35 (COOH-D-Y-M-G-W-M-D-F-NH2). Stock solution in NH4OH 0.05M and working solution in acetonitrile. I do MS infusion at conc. 500 ng/ml in...

22 June 2023 7,108 11 View

How to get a blank plasma cleared from amino acids?

I want to do validation but I need the same matrix of human plasma cleared from amino acids and other additives. I actually I thought of using buffer, what do think? Is it legal to use buffer for...

27 August 2021 6,133 5 View

Microarray for genotyping viral DNA in a blood sample?

To design a device with a microarray for genotyping viral DNA in a blood sample. The viral DNA sequence of the viral DNA which can be used for PCR-amplification has a length of 320 bp within 3...

31 July 2021 7,330 1 View

The quantifier for both analyte and internal standard have same product ion, will that have an impact on overall analysis?

Dear Chromatographers, This method was developed on LC MS/MS. The quantifier for both analyte and internal standard have same product ion, will that have an impact on overall analysis. Any...

31 August 2020 1,463 9 View

Non-consistent precursor ions in LC-MS/MS and reduced intensity after derivatization using TMS-DAM (peptide mass spec)?

Hi, I am trying to develop an assay to detect a conjugated peptide in plasma. I am essentially brand new to MS but have LC experience. The analyte of interest is a bisphosphonate tethered to...

12 January 2020 3,163 1 View

Is it OK to use AutoAnalyzer or BioAnalyzer for the measurement of cholesterol in bacterial culture media?

I would like to add cholesterol in bacterial growth media and determine cholesterol concentration in the presence and absence of some isolated bacteria. Is it OK to do it by the Auto-Analyzers /...

06 July 2019 9,715 1 View

Relative Response Factor Based On Internal Standard?

Dear to whom it may concern, I have just read the application of the relative response factor based on internal standard in analytical chemistry. I am much interested in this factoc. However, I...

04 May 2019 905 7 View

Can anybody tell the stability of blank biological matrix?

We have been doing a lot of bioanalysis of serum samples, for that there is no clarity about the expiry of blank biological matrix (basically serum) stored frozen. Is there any reference till how...

11 February 2019 3,877 5 View

Can anybody tell the stability of blank biological matrix?

We have been doing a lot of bioanalysis of serum samples, for that there is no clarity about the expiry of blank biological matrix (basically serum) stored frozen. Is there any reference till how...

01 February 2019 2,052 5 View