How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Does anyone use the ISE electrodes (ammonia, nitrate etc.) to characterise soils samples and plants extracts? What are advantages & disadvanteges?

I will have numerous soil samples/rice rhizosphere sample to characterise as part of the rice microbiome study. I was told that there are ISE electrodes that can be used to measure ammonia,...

19 July 2020 2,066 3 View

Which anti: GRP94 antibody do you recommend?

I am working with C.elegans strain which expresses H.s. GRP94 protein. I am looking for proper antibody to detect GRP94. Do you have some recommendation or suggestions?

13 September 2016 7,214 1 View

Do I need phylogenetic correction to my analyses when I have in data base 11 species?

Dear all, I am performing different analyses (ANOVA, ANCOVA, correlations etc.) and I am not sure if I need phylogenetic correction. On my dataset I have 11 closely related species from Carabidae...

22 November 2015 5,406 12 View

I'm looking for cell dye, which changes it's colour when entering the cell. Can anyone help?

18 October 2015 6,368 3 View

Does anyone have experience with polytypes of CdI2 and their structural calculations?

Or maybe know someone who has experience in this field?

08 February 2015 4,806 3 View

How can I analyse fluorescent images of Double/triple positive cells?

Hi I am using a Columbus for fluo images analysis. I need to define double or triple positive cells. My nuclei are stained with Hoestch (all cells), Sytox (dead cells only) and FITCs (apoptotic...

05 January 2015 3,830 4 View

Does anyone know how to soften the eyeball for histology (paraffin technique)?

02 December 2014 7,688 3 View

Can anyone help me to improve my Lipase assay Kwon and Rhee?

Could anyone help me to improve my lipase assay. I need to study the lipase activity produced by Bacillus saliphilus isolated from alkaline waste lime. I try to apply the method by Kwon and Rhee....

16 September 2014 9,717 29 View

Does anyone know about an ELISA kit that would measure small amounts of human urocortin 2 (UCN2) in serum/plasma?

Detection range around 10-20pg/ml. I have used one but it didn't even detect UCN2 added to the wells. Thanks.

20 May 2014 6,892 5 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

How to solve 2nd order non-linear and non-homogenous ordinary differential equation using non-linear shooting method and RK4 using Python?

I want to solve a second-order non-linear and non-homogenous order differential equation using the non-linear shooting method and fourth-order Runk Gutta using Python? Thank you for your help!

21 February 2021 3,225 3 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View

VSWR is shooting to 2.1 or even 2.3 in UHF dipole antenna, why?

So, I have a design, two dipoles working at UHF and VHF. We fed using a diplexer. I am having difficulties with the testing using network analyzer. VHF passes but UHF, specially at 400 MHZ, the...

26 January 2021 8,743 8 View

Which statistical test to use for comparing percentages between multiple groups?

Hello everyone! I am looking for some statistical help! I am working in in vitro micropropagation and one of the aspects that I am studying is the rooting of plants in 10 different growing media....

14 January 2021 7,575 5 View

Most of the plastic is shipped to Asia?

Dear Sir I came across your paper. First of all, congratulations on your amazing research article. Loved it! Also, I am very interested to know, why is the waste that is collected in the...

14 December 2020 6,342 4 View

Agmatin determination with LC MSMS?

I would like to determine agmatine (C5H14N4 CAS 306-60-5) with LCMSMS. The samples are sport supplements. The processing should be just dilute and shoot . I have not yet succeeded in achieving a...

29 November 2020 8,356 4 View