Hello,
I have recently switched from doing ligand into protein for ITC because my ligand is in DMSO and has low solubility at 10% DMSO; therefore, now I am doing protein into ligand -- the heat transfers are much better and seem to correlate to the protein concentration loaded in the syringe which is good to see.
However, at the end of my titration regardless of the concentration of ligand or protein added there is an increase in heat transfers. I am also seeing this in my protein injections into buffer alone.
Any thoughts on why this might be happening?
Joanna Sliwiak
Hi, what is the oligomeric state of your protein? Maybe there is a possibility that you observe some heat of protein association?
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