Nathan Matthieu Tang

4 Questions 1 Answers 0 Followers

Questions related from Nathan Matthieu Tang

Why are nucleoside analogs that are used to measure DNA replication always analogs of thymidine?

Analogs of thymidine such as BrdU or Tritated Thymidine are used to measure DNA synthesis because they can be labeled and they readily integrate into DNA, replacing thymidine. Analogs of other...

19 July 2017 4,373 2 View

How do you treat cells when measuring intracellular calcium?

I am currently working on measuring intracellular calcium levels when they are treated with Bapta-AM. I plated the cells at 40,000 cell/well/100 uL overnight in a 96 well plate and then loaded...

29 June 2017 9,856 2 View

What is the best media to use for culturing human foreskin fibroblasts?

I am using RPMI right now but I think DMEM will be better.

01 June 2017 2,542 1 View

What are the current problems with pseudo-typing viruses and their efficacy?

I was wondering what the limiting factors to pseudo-typing viruses in respect to treating cancer and selectively killing cells that need to be disposed of. A brief overview on how pseudo-typing...

10 May 2017 502 2 View