What is the best mechanical way to break the Rhizopus spore cell wall to obtain maximum RNA yield in case of low cell availability?

05 January 2024 0 6K Report

I have just 10^6 spores of Rhizopus in a sample. im using so fine glass pearls with Trizol and vortex about 10 min. but i cant see sharp bands of 28s,18s and 5s RNA in the agarose gel electrophoresis and just have a smear.

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Science method

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