Reza Ghaffaripour

3 Questions 2 Answers 0 Followers

Questions related from Reza Ghaffaripour

For cDNA synthesis from microRNA using a stem-loop primer, is preliminary process necessary before the main synthesis process,like heating the primer?

Well, as you know, the stem-loop primer has a long chain, increasing the likelihood of undesirable secondary structure formation.

05 January 2024 3,055 0 View

What is the most optimal temperature for cDNA synthesis from microRNA using stem-loop primer? Annealing and M-MLV cDNA synthesis Temprature?

Is there any difference between process time and temperature of cDNA synthesis using common primers (Oligo dT and Random Hexamer) compared with Stem-Loop primer of miRs?

05 January 2024 1,271 2 View

What is the best mechanical way to break the Rhizopus spore cell wall to obtain maximum RNA yield in case of low cell availability?

I have just 10^6 spores of Rhizopus in a sample. im using so fine glass pearls with Trizol and vortex about 10 min. but i cant see sharp bands of 28s,18s and 5s RNA in the agarose gel...

05 January 2024 5,610 0 View