Could you provide more details for the genera/species you are focused on?

KHANNA, Monisha; SOLANKI, Renu; LAL, Rup. Selective isolation of rare actinomycetes producing novel antimicrobial compounds. Int J Adv Biotechnol Res, 2011, 2. Jg., Nr. 3, S. 357-75.

ESHRAGHI, Seyyed Saeed. Molecular typing of Nocardia species. Journal of Medical Bacteriology, 2012, 1. Jg., Nr. 1.

http://www.biomedcentral.com/1471-2164/13/120

Thank you for your reply. Actually, the majarity of isolates belong to the genus Streptomyces, followed by Sacharomonospora and Nocardia.

Dear Mansooreh Jami

I am attaching the whole protocol for your kind consideration that i have for you. I am sure that it will solve most of your puzzles in your research.

(PCR Fingerprinting of rRNA Intergenic Spacer Regions for Molecular Characterization of Environmental Bacteria Isolates)

Best Regards

Dear Sibtain ,

thank you for your kind reply. I will go on your suggestion.

Hi,

Maybe this could be also interesting.

www.ncbi.nlm.nih.gov/pmc/articles/PMC3398770/?report=classic

https://www.researchgate.net/publication/13969334_Analysis_of_actinomycete_communities_by_specific_amplification_of_genes_encoding_16S_rRNA_and_gel-electrophoretic_separation_in_denaturing_gradients

http://www.aquaticbiosystems.org/content/pdf/2046-9063-8-23.pdf

Article Analysis of actinomycete communities by specific amplificati...