What is the most effective and simplest method for isolation of DNA from presumptive Actinobacteria?

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Any suggestions on the inconsistency between blast and phylogenetic tree?

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Any suggestions on full sequencing of 16S rRNA gene?

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Could anyone tell me if sequences derived from Actinobacteria primers are suitable for drawing phylogenetic trees?

I used the specific primers S-C-Act-0235-a-S-20 and S-C-Act-0878-a-A-18 in order to Identify actinomycetes. Do you think those sequence are enough to draw phylogenetic tree or I should use 8F and...

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What is the best genomic fingerprinting method for more identification of actinomycetes?

Could anybody tell me which molecular fingrprinting method would be the best for identification of actinomycetes in subspeices/strain level?

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Which primer set is the best one for the identification of Actinobacteria to the species level?

I used this primer set: SC-Act-235aS20, SC-Act-878aA19, but the results are good to the genus level but not to the species level.

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Do you think TSA could be a suitable media for purification of presumptive actinobacteria?

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What is a suitable fermentation broth for Actinomycete?

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How to store the presumptive isolation of Actinobacteria at -20?

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fatal error

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How to increase a model accuracy ?

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Christian Rückert

The protocol we usually use is attached.

Works for most Actinobacteria I tested so far (including Actinoplanes, Corynebacterium, Clavibacter, and Streptomyces).

Mansooreh Jami

Thank you for answer. Do you think could I use Proteinase K instead of Proteinase E?

Yes, that should work.

Dear Christina, in instruction is mentioned to resuspend the cells in water or TE buffer. As you know Actinobacteria are not suspend easily in water. Do you think is it better to put the colonies in broth or is there any way to use colonies directly.