Any suggestions on the inconsistency between blast and phylogenetic tree?

02 March 2015 9,727 13 View

Any suggestions on full sequencing of 16S rRNA gene?

I am trying to get full sequence of 16S rRNA gene from actinomycete isolates. I have tried 8F and 1492R set prime but I have got 1384 bps and is still lower than about 1490 bps. I was wondering if...

01 February 2015 5,840 5 View

Could anyone tell me if sequences derived from Actinobacteria primers are suitable for drawing phylogenetic trees?

I used the specific primers S-C-Act-0235-a-S-20 and S-C-Act-0878-a-A-18 in order to Identify actinomycetes. Do you think those sequence are enough to draw phylogenetic tree or I should use 8F and...

07 August 2014 8,341 2 View

What is the best genomic fingerprinting method for more identification of actinomycetes?

Could anybody tell me which molecular fingrprinting method would be the best for identification of actinomycetes in subspeices/strain level?

05 June 2014 9,555 5 View

Which primer set is the best one for the identification of Actinobacteria to the species level?

I used this primer set: SC-Act-235aS20, SC-Act-878aA19, but the results are good to the genus level but not to the species level.

02 March 2014 3,534 8 View

Do you think TSA could be a suitable media for purification of presumptive actinobacteria?

I isolated presumptive Actinobacteria from 3 media (Kuster agar, SCA, AIA) then put them in BHI broth at -80 . I am going to purify them on TSA.Do you think that could be a suitable media for all...

01 February 2014 7,749 7 View

What is the most effective and simplest method for isolation of DNA from presumptive Actinobacteria?

I am planning to extract DNA from presumtive Actinobacteria without using kit. Could anybody give me any idea of the most effective way to do that in order to get as much DNA as possible?

01 February 2014 6,019 4 View

Anyone know of what affect Cysteine-HCl has on culture media?

I added Cysteine-HCl to MRS and Colombia Blood Agar to reduce O2 of the media. could any body tell me should I store the media under anaerobic chamber because of Cysteine-HCl or it does not make...

31 December 2013 4,462 11 View

What is a suitable fermentation broth for Actinomycete?

I am going to use three different media (Actinomycete isolation agar, Starch Casein Agar, Glycerol Arginine Agar) for isolation of Actinobacteria from a freshwater ecosystem. Does anybody have any...

10 November 2013 4,653 31 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View