I have tested lipofectamine 2000, lipofectamine ltx and jet-Optimus in both case the efficacity of transfection is low and variable.
Dear Priscilla Doyon ,
The best DNA transfection agent for IDG-Sw3 cells will depend on several factors, including the specific characteristics of the cells, the type and amount of DNA being transfected, and the desired level of transfection efficiency.
One alternative transfection agent that has been used successfully in IDG-Sw3 cells is polyethylenimine (PEI). PEI is a positively charged polycationic polymer that forms complexes with negatively charged DNA and can effectively deliver DNA into cells. It is a highly efficient transfection reagent and is considered to be one of the most effective non-viral transfection agents. It is also relatively cheap, easy to use and has a high transfection efficiency.
Another alternative transfection agent that has been used successfully in IDG-Sw3 cells is Lipofectamine 3000 reagent, it is a novel transfection reagent that is designed to overcome the challenges associated with traditional transfection methods. It is a liposomal reagent that is designed to protect the DNA from degradation and to deliver it efficiently into the cells, it has been shown to be more efficient than lipofectamine 2000 and lipofectamine ltx in IDG-Sw3 cells.
It's important to note that the best transfection agent for IDG-Sw3 cells will depend on the specific experimental conditions and the goals of the research. It is recommended to try different transfection agents and to optimize the transfection conditions to achieve the best results.
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