Dear all,
I am currently researching about the antibacterial capability of certain proteins and protein hydrolizates against microbial cultures. We do not have any reference about the protein concentration of my matrix that could have antibacterial activity. Anyone could reccomend me what is the best dilution strategy for the determination of MIC from unknown proteins and protein hydrolizates?
Kind regards,
Start with the highest concentration that is feasible. Prepare about ten 2-fold serial dilutions from that.
Hello William
I agree with Adam; since you will have a stock solution of your protein, a good concentration to start would be a 1:10 dilution of it. To minimise the number of steps you'd have to perform to reach an MIC, you could use broad dilution-intervals. So concentrations of 1:10, 1:100, 1:1000 would be a good place to start. You can narrow it down later using 2-fold serial dilutions.
Thank you Adam and Shashank for your answers.
Kind regards,
William
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