Shashank Patole

3 Questions 4 Answers 0 Followers

Questions related from Shashank Patole

Why is the cDNA misbehaving in agarose gel?

This is bacterial cDNA created from an RNA prep which has been stored at -20ºC. It reads 100-150 ng/µl on a NanoDrop 2000, but run 3µl on a 2% Agarose (in 1xTAE) gel and all I get are blank...

07 November 2015 9,647 1 View

Can someone help me with the bacteria floating in wet mount making fluorescent imaging impossible?

I prepare wet mounts using 5 ul of stained, PFA-fixed bacterial suspensions per slide with a cover slip (22 x22 mm) placed on them. Despite allowing the slide to stand for 5 minutes, there is a...

29 August 2015 331 3 View

What concentration of FM dye do I need to stain Gram-negative bacteria?

I am using FM4-64 FX to stain Klebsiella bacterial cells.

27 August 2015 5,442 2 View