31 July 2023 1 1K Report

cytotoxicity test

More Arezoo Karamivandishi's questions See All
How long can PBMCs be stored at -70°C with good quality?

Can storing PBMCs at -70°C for a long time affect the population of PBMCs?

15 January 2024 8,010 3 View

Method of measuring sulfur for molybdenum oxide?

What is the method of measuring sulfur for minerals such as metal oxide or molybdenum oxide through Leco furnace (or similar furnaces) at different temperatures, times and weights after leaching...

11 November 2023 3,345 2 View

Nursing curriculum and educational system in canada and emarat countries?

i like to know some thing about nursing system in these country something like their salary and other things

06 December 2022 7,604 3 View

How to simulate turbines via EPANET toolkit in Python?

Hi researchers, I utilised epanet toolkit in Jupiter to model a micro pumped storage system. To model the turbine I substitute it with GPV. However, I faced an error when I was about assigning the...

08 November 2022 2,639 3 View

How can i find the rpm of my wind turbine in non rated speed?

my turbine is a variable speed wind turbine.

18 May 2022 7,250 4 View

Hi everyone. Does high concentrations of Tris-HCL buffer ( 2-3 mM Tris in 25 μl real time PCR reaction ) interfere with PCR reaction?

I have dissolved my primers in TE buffer 10X, which in the final PCR reaction will have an EDTA concentration of 0.02 mM and a Tris-HCL concentration of 2-3 mM. Will this interfere with the tag...

14 February 2022 6,667 3 View

How to get consistent result from CD spectroscopy?

I am doing CD studies on amino acids by measuring the CD intensity (quantative) for various concentrations of amino acids both when they are pure and when they have been used as precursors for...

07 December 2021 9,234 7 View

How can I measure electrical conductivity (EC) in mucilage seeds?

I want to measure EC in mucilage seeds. I do not want the seed coat to be damaged. So, how can I measure EC ? Is there another way to replace the electrical conduction method for mucilage seeds?

30 November 2021 6,539 5 View

How to differentiate the emission peak of a compound from the emission peak of the light source(instrument)?

I am having trouble recognizing whether the emission picks that I am seeing are from the light source of the instrument or from my sample?? is there a way to better discriminate them? (I am not...

21 November 2021 9,368 3 View

Hi everyone, Does anyone have any idea about my problem in macrophages polarization?

I am trying to polarize murine raw cells into 9 wells (C=6*10^4 and passage=7) with cytokines. I have used LPS and IFN-gamma for M1 and IL-10 and IL-4 for M2. But after polarization, the density...

28 October 2021 6,294 2 View

Similar questions and discussions
To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Hello, I wanted to know if it is a necessity to perform MTT test in three time points to evaluate the cytotoxicity of a metal alloy?

I am working on a magnesium based alloy, and i wanted to evaluate the cytotoxicity of my alloy with MTT assay. I want to know the reason why most of the researchers perform the MTT assay in three...

28 June 2024 2,570 1 View

Is there any role of alanine amino trasferase enzyme on cancer cell cytotoxicity?

Cell Cytotoxicity

24 June 2024 7,671 2 View

Discrepancy in Cytotoxicity Assessment of MDA-MB231 Cell Line: Seeking Clarification on IC50 Determination?

I'm currently conducting an experiment on the MDA-MB231 cell line, aiming to assess cytotoxicity using the XTT assay to determine the IC50. Initially, I seeded the cells at a density of 7000 cells...

14 April 2024 1,882 1 View

In a viability assay of a drug: How to determine if the toxicity of a product is due to its nature or to changes in osmolarity in the culture medium?

How to separate the cytotoxic effect of a molecule from that caused by the change in molarity at high concentrations of that molecule?

07 April 2024 3,745 0 View

Which LHD kit is better?

There are many LDH kits used for Cytotoxicity, such as Cytotoxicity LDH Assay Kit-WST (CK12, dojindo), CytoTox 96® Non-Radioactive Cytotoxicity Assay (G1780, Promega), and others. Could someone...

28 March 2024 4,989 1 View

Why does the cytotoxicity of herbal extract reduce at high concentrations?

We conducted SRB assay to survey the cytotoxicity of a herbal extract with the concentration from 0.5 to 18 mg/mL. However, the inhibition percent dramatically decrease at 15 mg/mL and 18 mg/mL...

26 March 2024 4,414 2 View

How to remove the preprint from researchgate?

Please, I urgently need to remove and delete this article preprint "Cytotoxicity and genotoxicity reveal the link between acute cadmium exposure and Alzheimer’s disease" from Researchgate. I can't...

13 March 2024 8,002 2 View

Is MTT assay feasible on Bacteria?

I have a question regarding the feasibility of using the MTT assay to measure the cytotoxic effects of Snail Mucin on E. coli and Pseudomonas. If this is indeed possible, what is the appropriate...

10 March 2024 9,935 3 View

Relationship between protein content and cytotoxicity results?

Hello everyone! I measured the protein concentration by BCA for each well of HeLa cells seeded in 6-well plate (750000 cells / 2ml DMEM per well). Protein concentration for each well was...

06 February 2024 7,499 2 View

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