Can storing PBMCs at -70°C for a long time affect the population of PBMCs?
I think -70 is the proper temperature for storage for long time in general.
Hello Arezoo Marjani,
Ideally, PBMCs should be cryopreserved in liquid nitrogen. When stored at - 70 degree C, PBMCs will show stable viability and function for up to 3 weeks. PBMCs may be used for several phenotypic and functional assays when stored at -70 degree C for less than 3 weeks after which you may expect decrease in viability and function.
You may want to refer to the article attached below for more information.
https://doi.org/10.1128%2FCVI.00342-08
Best.
Generally it is recommended to store PBMCs in liquid nitrogen if storing longer than 1 month. However, in my experience, PBMCs that we have stored in our -80C freezer have maintained their viability when stored for 2 years. I think that the stability of the cells can be impacted by many factors: quality of the cells before isolation/storage, the freezing media, the rate of freezing, how often the freezer is opened/closed (are the cells changing temperature every day?), and how the cells are thawed when using for an assay. It would be ideal to optimise the storage methods in your laboratory to suit your conditions.
Best of luck!
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