I need to achieve an insert size of 10Kb atleast
Hi Ruvini,
We've had great success at high quality DNA from our fungal strains using the plant kits from Qiagen. While the yield is typically low, the quality is excellent.
Has anyone used yeast nitrogen base (YNB) to make broth media at alkaline pH of 9-10? I tried buffering with HEPES, Tris, and Glycine but precipitation occurred all the time when the pH increased...
16 August 2023 806 0 View
My research study is an experimental study. My sample consist of 13 subjects (non random sampling). Can I find correlation between variables?
14 November 2017 8,969 5 View
If a questionnaire includes positively and negatively-keyed items, 1)should all the negatively keyed items be reverse coded? for example, if there are two items, 1. I like the game 2. The game is...
13 September 2017 5,190 10 View
I want find out a validated questionnaire to measure the relationship between graduate students engagement towards learning/research work and their academic performance. Can anyone help...
30 May 2017 9,882 2 View
I did a survey using google forms. I imported data from excel to SPSS. I wanted to find frequencies for some MCQs. There are more than one answer for my MCQs. But the output shows clusters of...
30 July 2016 7,176 9 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View